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Alice calls him Onii-chan in both the flashbacks and even the monster turned Alice is waiting for an Onii-chan. It was definitely a male. She forgot him when she turned into a monster, you can see her struggling to remembering him when she first starts transforming. Even when she saw him, she couldn't recognize him. Seeing the book they used to read together reminded Alice who it was, and when she cries, we see only Hakuno reflected in her eyes. In the ending, she again calls him Onii-chan, remembering who he is.